Anti biotics create substantial blood born debris.
This creates yet another problem on the path to survival
Numerous sites did post this break through,on one level or another,for about 2 days.
Regardless, this technology is clearly moving forward toward very practical application.
Our dependence upon antibiotics will have to end sooner than later.
They are also very messy, often creating their own systemic problems.
Several world health announcements in the last year seem to indicate that we have reached
the point of "Peak Antibiotic Effectiveness" TDNorth
Nature Medicine | Technical Report
This creates yet another problem on the path to survival
Numerous sites did post this break through,on one level or another,for about 2 days.
Regardless, this technology is clearly moving forward toward very practical application.
Our dependence upon antibiotics will have to end sooner than later.
They are also very messy, often creating their own systemic problems.
Several world health announcements in the last year seem to indicate that we have reached
the point of "Peak Antibiotic Effectiveness" TDNorth
Nature Medicine | Technical Report
An extracorporeal blood-cleansing device for sepsis therapy
- Nature Medicine
- doi:10.1038/nm.3640
- Received
- Accepted
- Published online
Abstract
Here we describe a blood-cleansing device for sepsis therapy inspired by the spleen, which can continuously remove pathogens and toxins from blood without first identifying the infectious agent.
Blood flowing from an infected individual is mixed with magnetic nanobeads coated with an engineered human opsonin—mannose-binding lectin (MBL)—that captures a broad range of pathogens and toxins without activating complement factors or coagulation.
Magnets pull the opsonin-bound pathogens and toxins from the blood; the cleansed blood is then returned back to the individual.
The biospleen efficiently removes multiple Gram-negative and Gram-positive bacteria, fungi and endotoxins from whole human blood flowing through a single biospleen unit at up to 1.25 liters per h in vitro.
In rats infected with Staphylococcus aureus or Escherichia coli, the biospleen cleared >90% of bacteria from blood, reduced pathogen and immune cell infiltration in multiple organs and decreased inflammatory cytokine levels.
In a model of endotoxemic shock, the biospleen increased survival rates after a 5-h treatment.
Read the full article
Supplementary Information links
Video
-
- Video 1: The fabrication and the magnetic separation principle of the biospleen: (2.6 MB, Download)
- Schematic drawing and microscopic video showing how the
biospleen device is fabricated and how the magnetically opsonized
pathogens are separated from the blood channel under flow.
Because it is difficult to observe the cell movement across the blood channel in the biospleen device, we demonstrated this in a microfluidic device fabricated from optically clear poly(dimethylsiloxane) (PDMS).
To mimic pathogens captured by the magnetic opsonins, fluorescent magnetic particles (8 μm, 1.1g ml–1, UMC4F, Bang Laboratories, Inc., IN, USA) were spiked into human banked blood (1ml) and flowed at 10 μl min–1. PDF files
-
- Supplementary Text and Figures (8,828 KB)
- Supplementary Figures 1–5, Supplementary Table 1
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